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The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice
Shota Yoshida 1 2, Hironori Nakagami 3, Hiroki Hayashi 2, Yuka Ikeda 4, Jiao Sun 2, Akiko Tenma 4, Hideki Tomioka 4, Tomohiro Kawano 2 5, Munehisa Shimamura 2, Ryuichi Morishita 6, Hiromi Rakugi 1
Abstract:
...Senescent T cells, defined as CD4+ CD44high CD62Llow PD-1+ CD153+ cells, accumulate in visceral adipose tissues (VAT) in obese individuals. Here, we show the long-lasting effect of using CD153 vaccination to remove senescent T cells from high-fat diet (HFD)-induced obese C57BL/6J mice. We administered a CD153 peptide-KLH (keyhole limpet hemocyanin) conjugate vaccine with Alhydrogel (CD153-Alum) or CpG oligodeoxynucleotide (ODN) 1585 (CD153-CpG) and confirmed an increase in anti-CD153 antibody levels that was sustained for several months. After being fed a HFD for 10-11 weeks, adipose senescent T cell accumulation was significantly reduced in the VAT of CD153-CpG-vaccinated mice, accompanied by glucose tolerance and insulin resistance. A complement-dependent cytotoxicity (CDC) assay indicated that the mouse IgG2 antibody produced in the CD153-CpG-vaccinated mice successfully reduced the number of senescent T cells. The CD153-CpG vaccine is an optional tool for senolytic therapy.
PMID: 32424156
Free Full-Text: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235045/
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