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Single Cell Analysis of Mitochondrial DNA Deletions
Methods Mol Biol. 2023;2615:443-463. doi: 10.1007/978-1-0716-2922-2_29.
Helen A L Tuppen # 1, Amy K Reeve 1, Amy E Vincent # 2
Abstract:
...Here, we detail protocols for laser micro-dissection and single cell lysis from tissues, and the subsequent analysis of deletion size, breakpoints and mutation load using long-range PCR, mtDNA sequencing and real-time PCR, respectively.
PMID: 36807808
Tags: methods, mtDNA, mtDNA deletions, mtDNA mutations