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One-step rapid tracking and isolation of senescent cells in cellular systems, tissues, or animal models via GLF16
Sophia Magkouta 1, Dimitris Veroutis 2, Athanasios Pousias 3, Angelos Papaspyropoulos 2, Kety Giannetti 4, Natassa Pippa 5, Nikolaos Lougiakis 3, Konstantinos Kambas 6, Nefeli Lagopati 7, Aikaterini Polyzou 8, Maria Georgiou 3, Maria Chountoulesi 9, Stergios Pispas 10, Spyros Foutadakis 11, Efthymios Kyrodimos 12, Nicole Pouli 3, Panagiotis Marakos 3, Athanassios Kotsinas 8, Panayotis Verginis 13, Dimitrios Valakos 11, Giannis Vatsellas 14, Russell Petty 15, Dimitris Thanos 16, Marco Demaria 17, Konstantinos Evangelou 8, Raffaella Di Micco 4, Vassilis G Gorgoulis 18
Abstract:
Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al.1.
PMID: 38460134
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