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Methods to monitor and compare mitochondrial and glycolytic ATP production.
Patergnani S, Baldassari F, De Marchi E, Karkucinska-Wieckowska A, Wieckowski MR, Pinton P
Abstract:
.....Here, we summarize conventional methods for measuring intracellular ATP levels and we provide a detailed protocol based on cytosol- and mitochondrion-targeted variants of firefly luciferase to determine the relative contribution of glycolysis and OXPHOS to ATP synthesis.
PMID: 24862273
SENS Approach:
