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Manipulation of mitochondrial genes and mtDNA heteroplasmy
Sandra R Bacman 1, P A Gammage 2, M Minczuk 3, Carlos T Moraes 4
Abstract:
...In the last decade, several enzyme-based gene editing platforms were developed to cleave specific DNA sequences. We have taken advantage of these enzymes to develop reagents to selectively eliminate mutant mtDNA. The replication of intact mitochondrial genomes normalizes mtDNA levels and consequently mitochondrial function. In this chapter, we describe the methodology used to design and express these nucleases in mammalian cells in culture and in vivo.
PMID: 32183972
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