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Identification of small juvenile stem cells in aged bone marrow and their therapeutic potential for repair of ischemic heart.
Am J Physiol Heart Circ Physiol. 2013 Aug 30. [Epub ahead of print] doi:
Igura K, Okada M, Kim HW, Ashraf M
Abstract:
.....In this study, we identified a novel sub-population of small juvenile stem cells (SJSCs) isolated from aged bone marrow stem cells (BMSCs) with high proliferation and differentiation potential. SJSCs expressed mesenchymal stem cell markers, CD29+/CD44+/CD59+/CD90+, but negative for CD45-/CD117- as examined by flow cytometry analysis. SJSCs showed higher proliferation, colony formation and differentiation abilities as compared to BMSCs. We also observed that SJSCs significantly expressed cardiac lineage markers (Gata-4 and Mef2C) and pluripotency markers (Oct-4, Sox-2, SSEA-1 and Nanog) as well as anti-aging factors such as Telomerase reverse transcriptase and Sirtuin1. Interestingly, SJSCs either from young or aged animals showed significantly longer telomere length as well as lower senescence-associated β-galactosidase expression, suggesting that SJSCs possess anti-aging properties while aged BMSCs have limited potential for proliferation and differentiation. Furthermore, transplantation of aged SJSCs into the infarcted rat heart significantly reduced the infarction size and improved left ventricle functions while aged BMSCs transplantation were less effective. Moreover, neovascularization as well as cardiomyogenic differentiation in peri-infarct area were significantly increased in SJSCs transplanted group as compared to BMSCs group, as evaluated by immunohistochemical analysis.....
PMID: 23997098
Tags: BMSC, SJSC, stem cells