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High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes
Lilian Vincis Pereira Sanglard 1, Catherine Colas des Francs-Small 2
Abstract:
Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.
PMID: 34545490
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