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Efficient Elimination of mtDNA from Mammalian Cells with 2',3'-Dideoxycytidine
DNA (Basel). 2024 Sep;4(3):201-211. doi: 10.3390/dna4030013.
Natalya Kozhukhar 1, Mikhail F Alexeyev 1
Abstract:
...Here, we examined the utility of 2',3'-dideoxycytidine (ddC, zalcitabine) alone or in combination with EtBr for generating ρ0 cell lines of mouse and human origin as well as inducing the ρ0 state in mouse/human somatic cell hybrids. We report that ddC is superior to EtBr in both immortalized mouse fibroblasts and human 143B cells. Also, unlike EtBr, ddC exhibits no cytostatic effects at the highest concentration tested (200 μM), making it more suitable for general use. We conclude that ddC is a promising new tool for generating mammalian ρ0 cell lines.
PMID: 39035221
Tags: methods, mtDNA, mtDNA depletion