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Simultaneous Imaging and Flow-cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-induced Senescent Cancer Cells
Eva Dovjak 1, Mario Mairhofer 1, Claudia Wöß 1, Jingjing Qi 1, Dorothy N Y Fan 2, Clemens A Schmitt 3, Yong Yu 4
Abstract:
...This paper presents a method to visualize and detect therapy-induced senescence (TIS) in cancer cells. Diffuse large B-cell lymphoma (DLBCL) cell lines were treated with mafosfamide (MAF) or daunorubicin (DN) and examined for the senescence marker, senescence-associated β-galactosidase (SA-β-gal), the DNA synthesis marker 5-ethynyl-2'-deoxyuridine (EdU), and the DNA damage marker gamma-H2AX (γH2AX). Flow cytometer imaging can help generate high-resolution single-cell images in a short period of time to simultaneously visualize and quantify the three markers in cancer cells.
PMID: 35913198
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