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Purification of Functional F-ATP Synthase from Blue Native PAGE.
Methods Mol Biol. 2019;1925:233-243. doi: 10.1007/978-1-4939-9018-4_20
Galber C, Valente G, von Stockum S, Giorgio V
Abstract:
In the presence of Ca2+, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.
PMID: 30674031
Tags: ATP synthase, methods, OXPHOS