A. Abramovich, N. Aizenberg, A. Luria, A. Braiman, H. Yanai, M. Wolfson, V.E. Fraifeld

Induction of Pluripotency (iP) has been shown to produce autologous stem cells that are viable for cell replacement therapy, with potential rejuvenative effect. Yet, the biological consequences of this process or iP-associated factors have scarcely been explored. Wound healing (WH) in adults, unlike early embryos, is usually a non-regenerative process in which the end-result is scar formation and a reduction in tissue function. iP and associated factors may have implications on the pathway of wound healing (e.g., scar-free or scar-formation). We suggest that iP-associated factors (genes and/or low-molecular weight compounds) may modulate WH due to the global or partial epigenetic reprogramming. This was based on our preliminary results on primary cultures of human fibroblasts -- in vitro model of WH, showing that (i) the fibroblasts which participate in WH, express stem cell markers; (ii) some of the iP factors have a positive modulatory effect on WH; (iii) pre-senescent fibroblast cultures retain the ability to respond to stimulants though differ from young fibroblasts in reaction degree. The in vitro WH model could be used as a test system for assessing the possible rejuvenative effect of the iP factors.

Keywords (Optional): 
wound healing
induction of pluripotency